肌肌酸激酶(CKM)重组蛋白(Rat,大鼠) 

Recombinant Creatine Kinase, Muscle (CKM)

CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed

[ PROPERTIES ]

Host: E.coli

Residues: Lys11~Leu367 (Accession # P00564),with N-terminal His-Tag

Tags: N-terminal His Tag

Subcellular Location: Cytoplasm

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.6

Predicted Molecular Mass: 41.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.MGHHHHHHSGSEF -KLNYKPQEEY PDLSKHNNHM AKVLTPDLYN KLRDKETPSGFTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYTVFKDLFD PIIQDRHGGY KPTDKHKTDLNHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFKGKYYPLKSMT EQEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVNEEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNEHLGYV LTCPSNLGTGLRGGVHVKLA NLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GAVFDISNAD RLGSSEVEQVQLVVDGVKLM VEMEKKL

[ IDENTIFICATION ]

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