糖原磷酸化酶L(PYGL)重组蛋白 (Rat,大鼠3) 

Recombinant Glycogen Phosphorylase, Liver (PYGL)

GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI

[ DESCRIPTION ]

Protein Names: Glycogen Phosphorylase, Liver

Synonyms: PYGL, Lgp

Species: Rat

Size: 100μg

Source: Escherichia coli-derived

Subcellular Location: C

[ PROPERTIES ]

Residues: Gln73~Val267 , withN-terminal His-Tag.

Purity: >95%, 26kDa as determined bySDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

 Endotoxin Level: <1.0 EU per 1μg (determined bythe LAL method)

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

 Predicted Molecular Mass: 23.6kDa

Predicted isoelectric point: 5.2

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37°C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGS- QHYYDKCP KRVYYLSLEF YMGRTLQNTM INLGLQNACD EAIYQLGLDMEELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEYGIFNQK IREGWQVEEADDWLRHGNPW EKARPEFMLP VHFYGRVEHT QAGTKWVDTQ VVLALPYDTPVPGYMNNTVN TMRLWSARAP NDFNLQDFNV GDYIQAV

[ IDENTIFICATION ]

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