胰岛素受体(INSR)重组蛋白(Rat,大鼠) 

Recombinant Insulin Receptor (INSR)

CD220; ISR; HHF5; IR

[ PROPERTIES ]

Host: E.coli

Residues: Ser1114~Pro1382 (Accession # P15127),with N-terminal His-Tag.

Subcellular Location: Cell membrane;Single-pass type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 32.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-SHLRSLR PDAENNPGRP PPTLQEMIQM TAEIADGMAY LNAKKFVHRDLAARNCMVAH DFTVKIGDFG MTRDIYETDY YRKGGKGLLP VRWMSPESLK DGVFTASSDMWS F GV V LW EI TS L A E Q P Y Q G LS N E QV L K F V MD G G Y L DP P D NCP E R LT D LMRMCWQFNPKM RPTFLEIVNL LKDDLHPSFP EVSFFYSEEN KAPESEELEMEFEDMENVPLDRSSHCQREE AGCREGGSSL SIKRTYDEHI PYTHMNGGKK NGRVLTLPRSNP

[ IDENTIFICATION ]

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