磷酸肌醇-3-激酶催化亚基β肽(PIK3Cb)重组蛋白(Human,人3) 

Recombinant Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)

PI3K; PI3Kbeta; PIK3C1; p110-BETA; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta

[ PROPERTIES ]

Source: Prokaryotic expression. 

Host: E. coli

Residues: Arg321~Pro507

Tags: N-terminal His-Tag

Subcellular Location: Cytoplasm. Nucleus. 

Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. 

Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.7

Predicted Molecular Mass: 22.7kDa

Accurate Molecular Mass: 23&28kDa as determined by SDS-PAGE reducing conditions. 

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. 

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

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