集钙蛋白2(CASQ2)重组蛋白 (Mouse,小鼠) 

Recombinant Calsequestrin 2 (CASQ2)

PDIB2; Cardiac Muscle Calsequestrin; Calsequestrin, cardiac muscle isoform

[ PROPERTIES ]

Accession: O09165

Host: E.coli

Residues: Glu35~Glu249

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Sarcoplasmic ReticulumLumen. Sarcoplasmic Reticulum Membrane;Peripheral Membrane Protein; Lumenal Side.Mitochondrion Matrix.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 4.2

Predicted Molecular Mass: 4.2

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

EDGLDF PEYDGVDRVI NVNAKNYKNV FKKYEVLALL YHEPPEDDKA SQRQFEMEELILELAAQVLE DKGVGFGLVD SEKDAAVAKK LGLTEEDSVY VFKGDEVIEY DGEFSADTLVEFLLDVLEDP VELIEGEREL QAFENIEDEI KLIGYFKSKD SEHYKAYEDA AEEFHPYIPFFATFDSKVAK KLTLKLNEID FYEAFMEEPM TIPDKPNSE

[ IDENTIFICATION ]

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