分离蛋白1(SCRN1)重组蛋白(Human,人) 

Recombinant Secernin 1 (SCRN1)

SES1

[ PROPERTIES ]

Residues: Ala2~Lys414

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q12765

Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 4.7

Predicted Molecular Mass: 50.0kDa

Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH?O.

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