胰岛素样蛋白5(INSL5)重组蛋白(Mouse,小鼠) 

Recombinant Insulin Like Protein 5 (INSL5)

[ PROPERTIES ]

Residues: Arg23~Cys135

Host: E.coli

Accession: Q9WUG6

Tags: Two N-terminal Tags, His-tag and GST-tag

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.6

Predicted Molecular Mass: 45.6kDa

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.RQTVKLCG LDYVRTVIYI CASSRWRRHL EGHFHSQQAE TRNYLQLLDR HEPSKKTLEHSLPKTDLSGQ ELVRDPQAPK EGLWELKKHS VVSRRDLQAL CCREGCSMKE LSTL

[ REFERENCES ]

1. Conklin D., et al. (1999) Genomics 60:50-56.

2. Hsu S.Y. (1999) Mol. Endocrinol. 13:2163-2174.

3. Dun S.L., et al. (2006) Endocrinology 147:3243-3248.

4. Burnicka-Turek O., et al. (2012) Endocrinology 153:4655-4665.

[ IDENTIFICATION ]

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