异连蛋白结合蛋白1(DTNBP1)重组蛋白(Human,人) 

Recombinant Dystrobrevin Binding Protein 1 (DTNBP1)

DBND; HPS7; My031; SDY; Dysbindin; Biogenesis of lysosome-related organelles complex 1 subunit 8; Hermansky-Pudlak syndrome 7 protein

[ PROPERTIES ]

Source: Prokaryotic expression. 

Host: E. coli

Residues: Gln100~Ser351

Tags: N-terminal His-Tag

Tissue Specificity: Placenta, Brain, Lung. 

Subcellular Location: Cell Junction, cell Membrane, cytoplasmic Vesicle, endoplasmic Reticulum, endosome, membrane, nucleus. 

Purity: >93%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 4.3

Predicted Molecular Mass: 31.9kDa

Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions. 

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 

2. Relative charge: The composition of amino acids may affects the charge of the protein. 

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 

5. Polymerization of the target protein: Dimerization, multimerization etc. 

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 

Store at 2-8℃ for one month. 

Aliquot and store at -80℃ for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. 

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

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