外核苷酸焦磷酸酶/磷酸二酯酶1(ENPP1)重组蛋白(Human,人2) 

Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1)

M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1; Membrane component chromosome 6 surface marker 1; Alkaline phosphodiesterase I; Plasma-cell membrane glycoprotein PC-1

[ PROPERTIES ]

Host: E.coli

Residues: Gly625~Leu780 , with N-terminal His-Tag.

Tags: N-terminal His Tag

Subcellular Location: Membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.5

Predicted Molecular Mass: 19.4kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGS- GCSCNP SILPIEDFQT QFNLTVAEEK IIKHETLPYG RPRVLQKENT

ICLLSQHQFM SGYSQDILMP LWTSYTVDRN DSFSTEDFSN CLYQDFRIPL SPVHKCSFYK

NNTKVSYGFL SPPQLNKNSS GIYSEALLTT NIVPMYQSFQ VIWRYFHDTL

[ IDENTIFICATION ]

[ REFERENCES ]

1. Buckley M.F., et al. (1990) J. Biol. Chem. 265:17506-17511.

2. Funakoshi I., et al. (1992) Arch. Biochem. Biophys. 295:180-187.

3. Pizzuti A., et al. (1999) Diabetes 48:1881-1884.

4. Belli S.I., Goding J.W. (1994) Eur. J. Biochem. 226:433-443.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。