视黄醇结合蛋白4(RBP4)重组蛋白(Gallus,鸡) 

Recombinant Retinol Binding Protein 4 (RBP4)

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

[ PROPERTIES ]

Host: E.coli

Residues: Glu22~Ser196 , with N-terminal His-Tag.

Tags: N-terminal His Tag

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 21.8kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: 

 The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-ERDCRVSSF KVKENFDKNR YSGTWYAMAK KDPEGLFLQD

NV VA Q F T V DE NG QM S ATA K G RV R L F N NW DV CA DM I GS F T D TE D PA K F KMK

YW GVA SF LQK GN D D HWV V D T DY D TYA L HY S CRE L NE D G T C ADS YS F VF S R

DPKGLPPEAQ KIVRQRQIDL CLDRKYRVIV HNGFCS

[ IDENTIFICATION ]

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