磷酸肌醇-3-激酶2α肽(PIK3C2a)重组蛋白 (Mouse,小鼠) 

Recombinant Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a)

PI3K2a; CPK; PI3-K-C2(Alpha); PI3-K-C2A; PI3K2-a; Phosphoinositide 3-Kinase 2a; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

[ PROPERTIES ]

Host: E.coli

Residues: Lys349~Pro611,with two N-terminal Tags, His-tag and GST-tag.

Subcellular Location: Cell membrane. Golgiapparatus. Cytoplasmic vesicle, clathrin-coatedvesicle. Nucleus. Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 61.9kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-KA QGQVSQKDPNGTSSLPTGSS LLQEFEVQND EVAAFCQSIM KLKTKFPYTD HCTNPGYLLS PVTVQRNMCGENASVKVSIE IEGLQLPVTF TCDVSSTVEI IIMQALCWVH DDLNQVDVGS YILKVCGQEEVLQNNHCLGS HEHIQNCRKW DTEIKLQLLT LSAMCQNLAR TAEDDEAPVDLNKYLYQIEKPYKEVMTRHP VEELLDSYHY QVELALQTEN QHRAVDQVIK AVRKICSALD GVETPSVTEAVKKLKRAVNL P

[ IDENTIFICATION ]

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