激活素A受体ⅡA(ACVR2A)重组蛋白(Mouse,小鼠) 

Recombinant Activin A Receptor Type II A (ACVR2A)

ACVR2-A; ACTRII; ACTR-II; ACVR2

[ PROPERTIES ]

Residues: Ala20~Lys95 linked with TALKCTFVAV

RAICVMKSPL IFRRWKSHSP LQILLHRSHP

DSSTTTTTTE IRLLTKPERK LSWLLPPLSN N

Tags: N-terminal His-Tag

Accession: P27038

Host: E. coli

Subcellular Location: Membrane; Single-pass type I membrane protein. 

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.6

Predicted Molecular Mass: 15.0kDa

Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

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