通用转录因子Ⅲ肽A(GTF3A)重组蛋白(Rat,大鼠) 

Recombinant General Transcription Factor IIIA (GTF3A)

AP2; TFIIIA

[ PROPERTIES ]

Accession: Q8VHT8

Host: E.coli

Residues: Val69~His363

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Nucleus.

Purity: >90% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 37.7kDa

Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducingconditions.

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VC DYE GC GKAFI RDY H LS RHI L IH TGEKP FV C ADN GC NQK FS TKS N LKK HI ERK HE NPQKQY VC NFEG CKKA FKK HQQ L RT H QCQ HT NEP LF RCT HE GC GK HFASPSRLKRH GKVHEGYLCQ KGCSFVGKTW TELLKHTREA HKEEVTCTVC QKMFKRKDHLKQHMKTHAPE RDVYRCPREG CARTYTTVFN LQSHILSFHE EKRPFVCEHA GCGKTFAMKQSLMRHSVVHD PDKKRMKLKV RPPRERRSLA SRLSGYVPPK GKQEPDCSLP NSTESSSSPEATMLAPAALL TVH

[ IDENTIFICATION ]

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