原始广泛存在蛋白1(AUP1)重组蛋白(Human,人)

Recombinant Ancient Ubiquitous Protein 1 (AUP1)

[ PROPERTIES ]

Accession: Q9Y679

Host: E.coli

Residues: Val201~Asp410

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Endoplasmic reticulum, Membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.7

Predicted Molecular Mass: 27.2kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test:  The thermal stability is described by the loss rate of thetarget protein. The loss rate was determined by accelerated thermal degradationtest, that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VSDASWVSEL LWSLFVPFTV YQVRWLRPVH RQLGEANEEF ALRVQQLVAK ELGQTGTRLTPADKAEHMKR QRHPRLRPQS AQSSFPPSPG PSPDVQLATL AQRVKEVLPHVPLGVIQRDLAKTGCVDLTI TNLLEGAVAF MPEDITKGTQ SLPTASASKF PSSGPVTPQP TALTFAKSSWARQESLQERK QALYEYARRR FTERRAQEAD

[ IDENTIFICATION ]

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