真核翻译起始因子3M(EIF3M)重组蛋白(Human,人) 

Recombinant Eukaryotic Translation Initiation Factor 3M (EIF3M)

PCID1; HFLB5; GA17; eIF3m; hfl-B5; PCI Domain Containing 1; Herpesvirus Entry Mediator; Fetal lung protein B5; PCI domain-containing protein 1

[ PROPERTIES ]

Residues: Ser2~Ser251

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q7L2H7

Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 31.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

SVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDVESVMNSVVSL LLILEPDKQE ALIESLCEKL VKFREGERPS LRLQLLSNLF HGMDKNTPVRYTVYCSLIKV AASCGAIQYI PTELDQVRKW ISDWNLTTEK KHTLLRLLYE ALVDCKKSDAASKVMVELLG SYTEDNASQA RVDAHRCIVR ALKDPNAFLF DHLLTLKPVK FLEGELIHDLLTIFVSAKLA S

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