干扰素诱导鸟苷酸结合蛋白1(GBP1)重组蛋白(Human,人) 

Recombinant Guanylate Binding Protein 1, Interferon Inducible (GBP1)

GTP-binding protein 1; Guanine nucleotide-binding protein 1

[ PROPERTIES ]

Residues: Thr35~Lys278

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P32455

Host: E. coli

Subcellular Location: Cytoplasm; Golgi Apparatus

Membrane; Lipid-anchor; Cytoplasmic Side; Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 31.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

TQPMVV VAIVGLYRTG KSYLMNKLA GKKKGFSLGS TVQSHTKGIWMWCVPHPKKPGHILVLLDTE GLGDVEKGD NQNDSWIFAL AVLLSSTFVY NSIGTINQQA MDQLYYVTELTHRIRSKSS PDENENEVED SADFVSFFPD FVWTLRDFSL DLEADGQPLT PDEYLTYSLKLKKGTSQKD ETFNLPRLCI RKFFPKKKCF VFDRPVHRRK LAQLEKLQD EELDPEFVQQVADFCSYIFS NSK

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