肌糖α(SGCa)重组蛋白(Human,人) 

Recombinant Sarcoglycan Alpha (SGCa)

SGC-A; A2; 50-DAG; ADL; DAG2; DMDA2; LGMD2D; SCARMD1; Adhalin; Dystroglycan-2; 50kDa Dystrophin-Associated Glycoprotein

[ PROPERTIES ]

Accession: Q16586

Host: E.coli

Residues: Gln24~His387

Tags: Two N-terminal Tags, His-tag and GST-tag

Subcellular Location: Cell Membrane; Single-passType I Membrane Protein. Cytoplasm.

Purity: >90% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 70.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

QQTTLHP LVGRVFVHTL DHETFLSLPE HVAVPPAVHI TYHAHLQGHP DLPRWLRYTQRSPHHPGFLY GSATPEDRGL QVIEVTAYNR DSFDTTRQRL VLEIGDPEGP LLPYQAEFLVRSHDAEEVLP STPASRFLSA LGGLWEPGEL QLLNVTSALD RGGRVPLPIE GRKEGVYIKVGSASPFSTCL KMVASPDSHA RCAQGQPPLL SCYDTLAPHF RVDWCNVTLV DKSVPEPADEVPTPGDGILE HDPFFCPPTE APDRDFLVDA LVTLLVPLLV ALLLTLLLAY VMCCRREGRLKRDLATSDIQ MVHHCTIHGN TEELRQMAAS REVPRPLSTL PMFNVHTGER LPPRVDSAQVPLILDQH

[ IDENTIFICATION ]

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