甘氨酰tRNA合成酶(GARS)重组蛋白(Human,人)

Recombinant Glycyl tRNA Synthetase (GARS)

CMT2D; CMT2-D; DSMAV; GlyRS; SMAD1; EJ; Charcot-Marie-Tooth Neuropathy 2D; Glycine tRNA Ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase

[ PROPERTIES ]

Source: Prokaryotic expression. 

Host: E. coli

Residues: Val567~Glu735

Tags: N-terminal His-Tag

Tissue Specificity: Brain, Spinal Cord. 

Subcellular Location: Mitochondrion. Cytoplasm. 

Purity: >93%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. 

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.1

Predicted Molecular Mass: 22.7kDa

Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions. 

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE

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