紧密连接蛋白3(TJP3)重组蛋白(Rat,大鼠) 

Recombinant Tight Junction Protein 3 (TJP3)

ZO-3; Zona Occludens 3; Zonula occludens protein 3

[ PROPERTIES ]

Accession: D3Z8G7

Host: E.coli

Residues: Met1~Ala243

Tags: Two N-terminal Tags, His-tag and T7-tag

Subcellular Location: Cell Junction. Cell Membrane.Membrane. Tight Junction.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 10.0

Predicted Molecular Mass: 29.8kDa

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MAVADMEELT IWEQHTATLY KDPRRGFGIA VSGGHDRAGG PVVVSDVVPGGPAEGRLQTGDHIVMVNGVS VENVTSAFAI QILKTCTKTA NITVKRPRRV QLPATKASPA SGHQLSDQEEADHGRGYEGD SSSGSGRSWG ERSRRSRAGR RVRMGSHGRR SSCGGSEANGLDLVSGYKRL PKQDVLMRPL KSVLVKRRNS EEFGVKLGSQ IFIKHITESGLAARNRGLQEGDLILQINGV SSA

[ IDENTIFICATION ]

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