谷胱甘肽S转移酶π1(GSTp)重组蛋白(Rat,大鼠) 

Recombinant Glutathione S Transferase Pi (GSTp)

GSTP1; GST-P1; DFN7; FAEES3; GST3

[ PROPERTIES ]

Host: E.coli

Residues: Met1~Gln210 ,with N-terminal His-Tag.

Subcellular Location: Cytoplasm. Mitochondrion.Nucleus.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 7.2

Predicted Molecular Mass: 24.7kDa

Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducingconditions.

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGS - MPPYTIVYFP VRGRCEATRM LLADQGQSWK EEVVTIDVWLQGSLKSTCLY GQLPKFEDGD LTLYQSNAIL RHLGRSLGLY GKDQKEAALV DMVNDGVEDLRCKYGTLIYT NYENGKDDYV KALPGHLKPF ETLLSQNQGG KAFIVGNQIS FADYNLLDLLLVHQVLAPGC LDNFPLLSAY VARLSARPKI KAFLSSPDHL NRPINGNGKQ

[ REFERENCES ]

1. Panasevich S., et al. (2010) Clin. Exp. Allergy 40:458-467.

2. Lakhdar R., et al. (2010) Genet. Mol. Res. 9:897-907.

3. Kim W.J., et al. (2011). Eur. Respir. J. 37:39-43

4. Reddy P., et al. (2010) Lung 188:409-414.

[ IDENTIFICATION ]

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