红细胞生成素受体(EPOR)重组蛋白(Mouse,小鼠) 

Recombinant Erythropoietin Receptor (EPOR)

EPO-R

[ DESCRIPTION ]

Protein Names: Erythropoietin Receptor

Synonyms: EPOR

Species: Mouse

Size: 100μg

Source: Escherichia coli-derived

Subcellular Location: Cell membrane; Single-pass type I membrane protein. Secreted.

[ PROPERTIES ]

Residues: Arg45~Ala244, with N-terminal His-Tag. 

Grade & Purity: >95%, 24 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). 

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 23.7 kDa

Predicted isoelectric point: 6.1

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-RGSEELLCFTQRLEDLVCFWEEAASSGMDFNYSFSYQLEGESRKSCSLHQAPTVRGSVRFWCSLPTADTSSFVPLELQVTEASGSPRYHRIIHINEVVLLDAPAGLLARRAEEGSHVVLRWLPPPGAPMTTHIRYEVDVSAGNRAGGTQRVEVLEGRTECVLSNLRGGTRYTFAVRARMAEPSFSGFWSAWSEPASLLTA

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