核因子κB激酶抑制因子相互作用蛋白(IKBIP)重组蛋白(Human,人)

Recombinant Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP)

IKIP; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Beta Interacting Protein

[ PROPERTIES ]

Residues: Trp132~Gln314,with two N-terminal Tags, His-tag and T7-tag. 

Host: E. coli

Subcellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 24.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WSNRITEKQDILNNSLTTLSQDITKVDQS TTSMAKDVGL KITSVKTDIR RISGLVTDVI SLTDSVQELE NKIEKVEKNTVKNIGDLLSS SIDRTATLRK TASENSQRIN SVKKTLTELK SDFDKHTDRF LSLEGDRAKVLKTVTFANDL KPKVYNLKKD FSRLEPLVND LTLRIGRLVT DLLQ

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