基质金属蛋白酶3(MMP3)重组蛋白(Rat,大鼠2) 

Recombinant Matrix Metalloproteinase 3 (MMP3)

SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Phe98~Cys475

Tags: N-terminal His Tag

Tissue Specificity: Kidney.

Subcellular Location: Secreted, extracellular space, extracellular matrix.

Purity: >90% 

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO₃, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 46.2kDa

Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO₃, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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