L选择素(SELL)重组蛋白 (Human,人) 

Recombinant Selectin, Leukocyte (SELL)

CD62L; L-Selectin; SEL-L; LAM1; LECAM1; LNHR; LSEL; LYAM1; Leu-8; Lyam-1; PLNHR; TQ1; HLHRc; MEL14; Lymphocyte Adhesion Molecule 1; Lymph Node Homing Receptor

[ PROPERTIES ]

Accession: P14151

Host: E.coli

Residues: Glu109~Ser346

Tags: Two N-terminal Tags, His-tag and GST-tag

Subcellular Location: : Membrane; Single-passtype I membrane protein.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 58.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

EE AENWGDGEPN NKKNKEDCVE IYIKRNKDAG KWNDDACHKL KAALCYTASCQPWSCSGHGE CVEIINNYTC NCDVGYYGPQ CQFVIQCEPL EAPELGTMDC THPLGNFSFSSQCAFSCSEG TNLTGIEETT CGPFGNWSSP EPTCQVIQCE PLSAPDLGIMNCSHPLASFSFTSACTFICS EGTELIGKKK TICESSGIWS NPSPICQKLD KSFSMIKEGDYNPLFIPVAVMVTAFS

[ REFERENCES ]

1. Siegelman M.H., Weissman I.L. (1989) Proc. Natl. Acad. Sci. U.S.A. 86:5562-5566.

2. Tedder T.F., et al. (1989) J. Exp. Med. 170:123-133.

3. Camerini D., et al. (1989) Nature 342:78-82.

4. Ord D.C., et al. (1990) J. Biol. Chem. 265:7760-7767.

[ IDENTIFICATION ]

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