血小板内皮细胞粘附分子1(PECAM1)重组蛋白(Rat,大鼠)

Recombinant Platelet/Endothelial Cell Adhesion Molecule (PECAM1)

CD31; EndoCAM; GPIIA'; PECA1

[ PROPERTIES ]

Host: E.coli

Residues: Arg459~Glu624 ,with two N-terminal Tags, His-tag and GST-tag.

Tags: N-terminal His Tag

Subcellular Location: Membrane; Single-passtype I membrane protein. Cell junction.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.7

Predicted Molecular Mass: 51.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-RD MEYQCIVDNCHSHPEVRSEI LRVKVIAPVD EVTISILSGN DVQSGDEMVL RCSVKEGTGP VTFQFYKEKEGRPFHEETVN DTQVFWHHEQ TSKEQEGQYY CTAFNRASIV TSLRSGPLTV RVFLAPWKKGLIAVVVIGVV IAALIVAAKY YFLRKAKAKQ KPVE

[ REFERENCES ]

1. Pepper M.S., et al. (2000) Dev. Dyn. 218:507-524. 2. Pinter E., et al. (1999) Am. J. Pathol. 154:1367-1379. 3. Wenzel K., et al. (1999) Hum. Mutat. 14:545-545. 4. Sasaoka T., et al. (2001) Ann. N. Y. Acad. Sci. 947:259-269.

[ IDENTIFICATION ]

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