肿瘤坏死因子配体超家族成员12(TNFSF12)重组蛋白(Human,人2) 

Recombinant Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12)

APO3L; DR3LG; TWEAK; APO3 ligand; TNF-related weak inducer of apoptosis

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Arg148~Gln247

Tags: N-terminal His Tag

Tissue Specificity: Heart, Pancreas, Brain, Spleen.

Subcellular Location: Cell membrane; Single-pass type II membrane protein, Secreted.

Purity: >98% 

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 12.4kDa

Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs fromthe predictedareasfollows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

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