细胞内卵巢癌抗原1(COVA1)重组蛋白(Mouse,小鼠) 

Recombinant Cytosolic Ovarian Carcinoma Antigen 1 (COVA1)

APK1; tNOX; ENOX2; Ecto-NOX Disulfide-Thiol Exchanger 2; Tumor-associated hydroquinone oxidase; Hydroquinone [NADH] oxidase; Protein disulfide-thiol oxidoreductase

[ PROPERTIES ]

Host: E.coli

Residues: Met1~Met207

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q8R0Z2

Subcellular Location: CellMembrane, membrane, secreted.

Purity: >95% 

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 26.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH?O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37°C for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MTLPVSDPAA WATAMNNLGM APLGIAGQPI LPDFDPALGM MTGIPPITPMMPGLGIVPPPIPPDMPVAKE IIHCKSCTLF PPNPNLPPPA TRERPPGCKT VFVGGLPENGTEQIIVEVFEQCGEIIAIRK SKKNFCHIRF AEEYMVDKAL YLSGYRIRLG SSTDKKDTGR LHVDFAQARDDLYEWECKQR MLAREERHRR RMEEERM

[ IDENTIFICATION ]

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