前列腺酸性磷酸酶(PAP)重组蛋白(Rat,大鼠)

Recombinant Prostatic Acid Phosphatase (PAP)

PSAP; ACPP; ACP3; PAPf39; 5'-NT; Prostatic Specific Acid Phosphatase; Ecto-5'-Nucleotidase

[DESCRIPTION ]

Protein Names: Acid Phosphatase 3, Prostatic

Synonyms: ACP3

Species: Rat

Size: 100μg

Source: Escherichia coli-derived

Subcellular Location: Secreted, Cell membrane; Single-pass type I membrane protein.

[ PROPERTIES ]

Residues: Val4~Ile418 , with N-terminal His-Tag.

Grade & Purity: >95%, 50 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). 

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 50.1 kDa

Predicted isoelectric point: 6.8

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test:  The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCES]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSG SEFELRRQAC GR-VPLHLVGT ASLTLGFLLL LSLRLDPGQA KELKFVTLVF RHGDRGPIET FPNDPIKESS

WPQGFGQLTK WGMGQHYELG SYIRRRYGRF LNNSYKHDQV YIRSTDVDRT LMSAMTNLAA LFPPEGISIW NPRLLWQPIP

VHTVSLSEDR LLYLPFRDCP RFQELKSETL KSEEFLKRLQ PYKSFIDTLP SLSGFEDQDL FEIWSRLYDP LYCESVHNFT

LPTWATEDAM TKLKELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILKNMK LATQPQKARK LIMYSAHDTT VSGLQMALDV

YNGLLPPYAS CHIMELYQDN GGHFVEMYYR NETQNEPYPL TLPGCTHSCP LEKFAELLDP VIPQDWATEC MGTSNHQVLR

VILATTFCLV TGILVILLLV LIRHGPCWQR DVYRNI

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