血管紧张素原(AGT)重组蛋白 (Rat,大鼠) 

Recombinant Angiotensinogen (AGT)

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

[ PROPERTIES ]

Residues: Asp25~Val477,with two N-terminal Tags, His-tag and S-tag.

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 55.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8℃ for one month.

Aliquot and store at -80℃ for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- DRVYIH

PFHLLYYSKS TCAQLENPSV ETLPEPTFEP VPIQAKTSPV DEKTLRDKLV LATEKLEAED

RQRAAQVAMI ANFMGFRMYK MLSEARGVAS GAVLSPPALF GTLVSFYLGS LDPTASQLQV

LLGVPVKEGD CTSRLDGHKV LTALQAVQGL LVTQGGSSSQ TPLLQSTVVG LFTAPGLRLK

QPFVESLGPF TPAIFPRSLD LSTDPVLAAQ KINRFVQAVT GWKMNLPLEG VSTDSTLFFN

TYV HFQGKMR GFSQ LTG L HE FWVDNSTSVS VPM LSGTGNF QHWS DAQNNF

SVTRVPLGES VTLLLIQPQC ASDLDRVEVL VFQHDFLTWI KNPPPRAIRL TLPQLEIRGS

YNLQDLLAQA KLSTLLGAEA NLGKMGDTNP RVGEVLNSIL LELQAGEEEQ PTESAQQPGS

PEVLDVTLSS PFLFAIYERD SGALHFLGRV DNPQNVV

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